Publications on Enzymes and History of Science
Papers on enzymes, including aspartate aminotransferase, and the history of biochemistry
Allocating papers to one category or another makes for some odd bed-fellows. History falls here, for convenience, and the enzyme alkaline phosphatase under 'calcification'.
Biochemistry came late to Hong Kong, in the sense of an independent university subject, and has now grown to maturity with flourishing departments in several universities.
The early years of biochemistry in Hong Kong, from 1965.
Pyridoxine supplements in the carpal tunnel syndrome. British Medical Journal 311 (1995) 700.
TRC Boyde ACB Newsletter 37C February 1994 Laboratory Diagnosis of MI and AST Determination.
Venkat Lakshman Rao PAPINENI (PhD, Hong Kong, 1993) (Supervisor). Physical status of mitochondrial aspartate aminotransferase in serum and the role of alpha-2 macroglobulin in its clearance.
papineni-phd
F. Monsanto, G. Ghislani, B.L. Blas, L. Tormis and T.R.C. Boyde, Mitochondrial aspartate aminotransferase. Serum levels in schistosomiasis japonica. Molecular Basis of Pathogenesis in Parasitic Diseases - Proceedings of the Second SE Asian Symposium on Parasitology and Modern Medicine, Hong Kong, December (1988)
Immunity and Schistosomiasis. Medicine Digest 13 (1987) 4-8. Reprinted in Medical Digest, Asia, vol 5.
The isoenzymes of aspartate transaminase differ in their kinetic properties in that the cytoplasmic isoenzyme is more readily inhibited by adipate and by 2-oxoglutarate (substrate) at low pH. A differential kinetic assay based on this phenomenon has been optimised for use in assays of serum samples. The new method agrees well with an immune absorption procedure. Methods based on chromatographic separation of the isoenzymes fail in the presence of serum.
E.M.L. Kwong and T.R.C. Boyde, Kinetic differential assay of aspartate aminotransferase isoenzymes in serum. Clin Chim Acta 128 (1983) 95-102
M. Rahmatullah and T.R.C. Boyde, Kinetics of ornithine carbamoyltransferase. Biochem Soc Trans 9 (1981) 454-5
T.R.C. Boyde, A non-enzymatic reaction that interferes with measurement of serum aspartate aminotransferase activity at pH 6. Clin Chem 27 (1981) 1783-4
Mohamed RAHMATULLAH (PhD, Hong Kong, 1981) (Supervisor) Kinetics and Assay of Ornithine Transcarbamyase